Lab Dna Restriction Enzyme Simulation Answer Key < ORIGINAL >

EcoRI cuts lambda at 5 sites: coordinates 21,226; 26,104; 31,747; 39,168; 44,972 (in the classic GenBank map). Therefore, 6 fragments (linear DNA with n cuts = n+1 fragments). Sizes: 21,226 – 0 = 21,226 bp; then 26,104-21,226 = 4,878; 31,747-26,104 = 5,643; 39,168-31,747 = 7,421; 44,972-39,168 = 5,804; and 48,502-44,972 = 3,530. Fragments in bp: 21,226; 7,421; 5,804; 5,643; 4,878; 3,530.

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Restriction enzymes, or restriction endonucleases, are proteins that cut DNA at specific sequences known as recognition sites. In nature, bacteria use these enzymes as a defense mechanism against viral DNA. In the lab, scientists use them to map genomes and create recombinant DNA. Key Concepts for Your Simulation Lab Dna Restriction Enzyme Simulation Answer Key